The new law could see drivers paying a £3.50 daily charge when going from Kent to London.
But Conservative MP Gareth Johnson attacked the proposals as he warns it'll put a "financial wall" between the cities.
He also told how the scheme would impact families, businesses and key public sector workers.
The new charge would affect the lowest-paid members of the community, the MP warned.
Mr Johnson said: "This proposal by the Mayor of London would put up a literal financial wall between Kent and London.
"If it goes ahead, any motorist would have to pay at least £3.50 seven days a week simply for moving from Kent into London.
"It cannot be allowed to happen, we have got to do everything we can to stop this because this is Sadiq Khan at his worst."
Dartford Conservatives teamed up with their colleagues in Bexley to protest against the proposals.
They even made banners urging drivers to "hoot to say no" and warned of "checkpoint Crayford".
Meanwhile, over 26,000 have already signed Dartford Conservatives' online petition against the new tax plans.
Peter Fortune, candidate for Bexley and Bromely, also warned the "bonkers" scheme could "hammer families".
Drivers warned swearing at others on the road could land them hefty fine
He said: "We are opposing Sadiq Khan's bonkers charge that would leave drivers paying £5.50 to drive into London.
"It is going to hammer our businesses and friends and families who are going about their daily lives and visiting relatives.
"The idea is crazy, it has to be stopped."
The London mayor has repeatedly said the charges will be introduced if he doesn't get access to the £500million in VED paid.
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However, Mr Khan's spokesperson said a £3.50 daily charge would reduce London's weekday car traffic by around 5%.
They said: "The Mayor has repeatedly urged the Government to allow London to retain the £500m of Vehicle Excise Duty paid by Londoners every year but which is currently spent almost exclusively on maintaining roads outside the capital.
"If the Government do not agree, other ways of raising money to overcome the unprecedented financial challenges TfL faces as a result of the Covid-19 pandemic may be needed.
"A Greater London Boundary Charge for non-residents could reduce congestion and emissions whilst encouraging more use of public transport. Revenues could also provide funding for investment in London's transport network."
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